SKIN GRAFTING

Skin grafting is a surgical procedure in which skin or a skin substitute is placed over a burn or non-healing wound. A skin graft is used to permanently replace damaged or missing skin or to provide a temporary wound covering. This covering is necessary because the skin protects the body from fluid loss, aids in temperature regulation, and helps prevent disease-causing bacteria or viruses from entering the body. Skin that is damaged extensively by burns or non-healing wounds can compromise the health and well-being of the patient.

Demographics
Although anyone can be involved in a fire and need a skin graft, the population groups with a higher risk of fire-related injuries and deaths include:
• children four years old and younger
• adults 65 years and older
• African Americans and Native Americans
• low-income Americans
• persons living in rural areas
• persons living in manufactured homes (trailers) or substandard housing

Description
The skin is the largest organ of the human body. It is also known as the integument or integumentary system because it covers the entire outside of the body. The skin consists of two main layers: the outer layer, or epidermis, which lies on and is nourished by the thicker dermis. These two layers are approximately 0.04–0.08 in (1–2 mm) thick. The epidermis consists of an outer layer of dead cells called keratinocytes, which provide a tough protective coating, and several layers of rapidly dividing cells just beneath the keratinocytes. The dermis contains the blood vessels, nerves, sweat glands, hair follicles, and oil glands. The dermis consists mainly of connective tissue, which is largely made up of a protein called collagen. Collagen gives the skin its flexibility and provides structural support. The fibroblasts that make collagen are the main type of cell in the dermis.

Skin varies in thickness in different parts of the body; it is thickest on the palms and soles of the feet, and thinnest on the eyelids. In general, men have thicker skin than women, and adults have thicker skin than children. After age 50, however, the skin begins to grow thinner again as it loses its elastic fibers and some of its fluid content. Injuries treated with skin grafts Skin grafting is sometimes done as part of elective plastic surgery procedures, but its most extensive use is in the treatment of burns. For first or second-degree burns, skin grafting is generally not required, as these burns usually heal with little or no scarring.

With third-degree burns, however, the skin is destroyed to its full depth, in addition to damage done to underlying tissues. People who suffer third-degree burns often require skin grafting. Wounds such as third-degree burns must be covered as quickly as possible to prevent infection or loss of fluid. Wounds that are left to heal on their own can contract, often resulting in serious scarring; if the wound is large enough, the scar can actually prevent movement of limbs. Non-healing wounds, such as diabetic ulcers, venous ulcers, or pressure sores, can be treated with skin grafts to prevent infection and further progression of the wounded area.

Types of skin grafts
The term “graft” by itself commonly refers to either an allograft or an autograft. An autograft is a type of graft that uses skin from another area of the patient’s own body if there is enough undamaged skin available, and if the patient is healthy enough to undergo the additional surgery required. An allograft uses skin obtained from another human being, Donor skin from cadavers is frozen, stored, and available for use as allografts. Skin taken from an animal (usually a pig) is called a xenograft because it comes from a nonhuman species. Allografts and xenografts provide only temporary covering because they are rejected by the patient’s immune system within seven days. They must then be replaced with an autograft.

SPLIT-THICKNESS GRAFTS. The most important part of any skin graft procedure is proper preparation of the wound. Skin grafts will not survive on tissue with a limited blood supply (cartilage or tendons) or tissue that has been damaged by radiation treatment. The patient’s wound must be free of any dead tissue, foreign matter, or bacterial contamination. After the patient has been anesthetized, the surgeon prepares the wound by rinsing it with saline solution or a diluted antiseptic (Betadine) and removes any dead tissue by débridement. In addition, the surgeon stops the flow of blood into the wound by applying pressure, tying off blood vessels, or administering a medication (epinephrine) that causes the blood vessels to constrict.

Following preparation of the wound, the surgeon then harvests the tissue for grafting. A split-thickness skin graft involves the epidermis and a little of the underlying dermis; the donor site usually heals within several days. The surgeon first marks the outline of the wound on the skin of the donor site, enlarging it by 3–5% to allow for tissue shrinkage. The surgeon uses a dermatome (a special instrument for cutting thin slices of tissue) to remove a split-thickness graft from the donor site. The wound must not be too deep if a split-thickness graft is going to be successful, since the blood vessels that will nourish the grafted tissue must come from the dermis of the wound itself. The graft is usually taken from an area that is ordinarily hidden by clothes, such as the buttock or inner thigh, and spread on the bare area to be covered. Gentle pressure from a well-padded dressing is then applied, or a few small sutures used to hold the graft in place. A sterile nonadherent dressing is then applied to the raw donor area for approximately three to five days to protect it from infection.